John (Mick) Robbins

Building: Petit IBB
Office: 3428
Phone: 404.385.3089
Fax: 404.894.2291
john [dot] robbins [at] chbe [dot] gatech [dot] edu (email)

Mailing Address

Georgia Institute of Technology
School of Chemical &
Biomolecular Engineering
311 Ferst Drive, N.W.
Atlanta, GA 30332-0100

Education

B.S. Chemistry, Hampden-Sydney College, 2007
Ph.D. Biochemistry, Auburn University, 2012

Research Interests

Biocatalysis

Publications

1. Robbins J.M., Gadda, G., Bommarius, A.S. (2018) Identification of Key Catalytic Amino Acid Residues in Formate Oxidase Catalysis. In preparation

2. Robbins J.M., Gadda, G., Bommarius, A.S. (2018) Importance of Light-Sensitive 8-Formyl FAD in Formate Oxidase Catalysis. In preparation

3. Robbins J.M., Ellis H.R. (2018) Implications of Rapid-Reaction Studies to Identify Intrinsic Solvent and Kinetic Isotope Effects for the Desulfonation Mechanism of Alkanesulfonate Monooxygenase. In preparation

4. Paye, Marietou; Rose, Harrison; Robbins, John; Yunda, Diana; Cho, Seonggeon; Bommarius, Andreas (2017) A high-throughput pH-based colorimetric assay: application focus on alpha/beta hydrolases. In review: Analytical Biochemistry

5. Robbins J.M., Gadda, G., Bommarius, A.S. (2017) Mechanistic Studies of Formate Oxidase from Aspergillus oryzae: A Novel Member of the Glucose-Methanol-Choline Oxidoreductase Enzyme Superfamily that Oxidizes Carbon Acids. In Review: Arch Biochem Biophys

6. Robbins J.M., Souffrant, M.G., Hamelberg, D., Gadda, G., Bommarius, A.S. (2017) Enzyme-Mediated Conversion of Flavin Adenine Dinucleotide (FAD) to 8-formyl FAD in Formate Oxidase Results in Modified Cofactor with Enhanced Catalytic Properties. Biochemistry 56 (29), 3800-3807

7. Robbins J.M., Ellis H.R. (2014) Steady-State Kinetic Isotope Effects Support Complex Role of Arg226 in Proposed Desulfonation Mechanism of the Two-Component Alkanesulfonate Monooxygenase System. Biochemistry 53 (1), 161-168.

8. Robbins J.M., Xiong J., Ellis, H.R., Alkane Sulfonate Monooxygenase and Related Systems. Handbook of Flavoproteins. Volume 2 Complex Flavoproteins, Dehydrogenases and Physical Methods. Hille, R., Miller, S., Palfey, B., Eds.; De Gruyter: Germany, 2013, p. 255-276.

9. Robbins J.M., Ellis H.R. (2012) Identification of Critical Steps Governing the Two-Component Alkanesulfonate Monooxygenase Catalytic Mechanism. Biochemistry 51 (32), 6378-6387

10. Robbins, J.M., Ellis, H.R. The Role of Flavin Intermediates in the Desulfonation Mechanism of the Two-Component Alkanesulfonate Monooxygenase System. In Proceedings of the 17th International Symposium of Flavins and Flavoproteins. Miller, S., Hille, R., Palfey, B., Cecchini, G. Eds.; Alan R. Liss: New York, 2011, p. 335-340.

11. Carpenter, R.A., Xiong J. Robbins J.M., Ellis H.R. (2011) Functional Role of Conserved Arginine Residue Located on a Mobile Loop of Alkanesulfonate Monooxygenase. Biochemistry 50 (29), 6469-6477.

Presentations

1. Robbins J.M., Gadda, G., Bommarius, A.S. (2017) Formate Oxidase (FOX) Activity is Governed by Autocatalytic Formation of 8-formyl FAD Exhibiting Unusual Properties. Vince Massey Award Oral Presentation, 19^th International Symposium on Flavins and Flavoproteins, Groningen, The Netherlands, July 3, 2017.

2. Robbins J.M., Gadda, G., Bommarius, A.S. (2016) Unusual 8-formyl-FAD Governs Activity of Formate Oxidase (FOX) from Aspergillus oryzae. Oral Presentation, SIMB Annual Meeting, New Orleans, LA, United States, July, 26 2016.

3. Robbins J.M., Gadda, G., Bommarius, A.S. (2016) Unusual 8-formyl-FAD Governs Activity of Formate Oxidase (FOX) from Aspergillus oryzae. Oral Presentation, Eighth Annual OxiZymes Meeting, Wageningen, The Netherlands, July, 5 2016.

4. Robbins J.M., Gadda, G., Bommarius, A.S. Mechanistic and Kinetic Implications of Unusual 8-formyl FAD in Formate Oxidase Catalysis. Poster presented at the 2015 Gordon Research Conference: Enzymes, Coenzymes, and Metabolic Pathways in Waterville Valley Resort, NH United States, July 12-17, 2015.

5. Robbins J.M., Gadda, G., Bommarius, A.S. Mechanistic and Kinetic Implications of Unusual 8-formyl FAD in Formate Oxidase Catalysis. Poster presented at the 2015 Gordon Research Seminar: Enzymes, Coenzymes, and Metabolic Pathways in Waterville Valley Resort, NH United States, July 11-12, 2015.

6. Robbins J.M., Gadda, G., Bommarius, A.S. (2015) Unusual 8-formyl-FAD Governs Activity of Formate Oxidase (FOX) from Aspergillus oryzae. Poster Talk, Sixth Annual Southeastern Enzyme Conference, Atlanta, GA, United States, April 11, 2015.